Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline
DOI:
https://doi.org/10.32539/bsm.v5i5.228Keywords:
ELISA, Antigen, Antibody, GuidelineAbstract
ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.
Authors
- Rachmat Hidayat1*
- Patricia Wulandari2
- 1Department of Biology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia
- 2Cattleya Mental Health Center, Palembang, Indonesia
Corresponding author Rachmat Hidayat — dr.rachmat.hidayat@gmail.com
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